PepR1, a CcpA-like transcription regulator of Lactobacillus delbrueckii subsp. lactis

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PepR1, a CcpA-like transcription regulator of Lactobacillus delbrueckii subsp. lactis.

The PepR1 protein from Lactobacillus delbrueckii subsp. lactis DSM 7290 shares extensive homology with catabolite-control proteins from various Gram-positive bacteria. Expression of the subcloned pepR1 gene allowed for partial complementation of a ccpA defect in Staphylococcus xylosus. The influence of PepR1 on transcription of the prolidase gene pepQ, which is located adjacent to pepR1, was ex...

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Autoregulation of the biosynthesis of the CcpA-like protein, PepR1, in Lactobacillus delbrueckii subsp bulgaricus.

PepR1 from Lactobacillus delbrueckii subsp bulgaricus (Lb. bulgaricus) is involved in biosynthesis regulation of the prolidase PepQ. In this paper, we demonstrated that Lb. bulgaricus PepR1 biosynthesis is not constitutive like those of several bacteria but is auto-regulated and depends on the glucose concentration of the culture medium. We propose a model for PepQ regulation by PepR1.

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Electrotransformation of Lactobacillus delbrueckii subsp. bulgaricus and L. delbrueckii subsp. lactis with various plasmids.

We describe, for the first time, a detailed electroporation procedure for Lactobacillus delbrueckii. Three L. delbrueckii strains were successfully transformed. Under optimal conditions, the transformation efficiency was 10(4) transformants per microg of DNA. Using this procedure, we identified several plasmids able to replicate in L. delbrueckii and integrated an integrative vector based on ph...

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Introduction of peptidase genes from Lactobacillus delbrueckii subsp. lactis into Lactococcus lactis and controlled expression.

Peptidases PepI, PepL, PepW, and PepG from Lactobacillus delbrueckii subsp. lactis, which have no counterparts in Lactococcus lactis, and peptidase PepQ were examined to determine their potential to confer new peptidolytic properties to lactococci. Controllable expression of the corresponding genes (pep genes) was achieved by constructing translational fusions with the promoter of the nisA gene...

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ژورنال

عنوان ژورنال: Microbiology

سال: 1999

ISSN: 1350-0872,1465-2080

DOI: 10.1099/00221287-145-11-3147